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Capillary Liquid Chromatography- Mass SpectrometryWhat is Chromatography? Chromatography is the technical border for a set of laboratory approaches for the separation of mixtures (Solid/Liquid/Gas). The mixture is change state in a fluid which called the mobile phase, which carries it through a coordinate holding another material known as the stationary phase. The mingled constituents of the mixture transport at different velocities, causing them to separate. The separation is primarily based on differential partitioning between the mobile and its stationary phases. Subtle differences in a compounds partition coefficient result in differential retention time on the stationary phase and thence changing the separation (Tomer, et al., 1994). Chromatography can be preparative or analytical. The basal purpose of preparative chromatography is to separate the components of a mixture for more groundbreaking uses (and also it is a method of purification). Analyti cal chromatography is done normally with little amounts of material and is for measuring the relative proportions of analytic in a mixture. The ii are not mutually exclusive (Covey, et al., 1986). Basically chromatography methods can categorize based on several factors. by chromatographic bed spirt1. Column chromatography2. Planner chromatography (Paper and Thin layer methods)Displacement Chromatographyby somatogenic state of mobile phase1.Gas2.Liquidby separation mechanism1.Adsorption chromatography2.Ion exchange chromatography3.Size exclusion chromatography4.Partition chromatography5. coincidence chromatographyFigure 1 Classification of Chromatography techniques. Liquid Chromatography Liquid chromatography (LC) is an analytical c... ... their plug / charge ratio (m/z). Several different techniques are available for twain ionization and ion analysis, resulting in many different catogories of mass spectrometers with different combinations of these devil processes. In practice, some combinations are far more versatile than others and the quest descriptions focus on the major types of ion sources and mass analyzers that used in LC-MS systems.Ion Sources1)Electrospray ionization Source2) atmospheric Pressure Chemical Ionization Source3)Atmospheric Pressure Photo IonizationMass Analyzers1)Quadruple Analyzers2)Time-of-flight Analyzers3)Ion Trap Analyzers4) interbreeding AnalyzersApplications of Capillary liquid chromatography- mass spectrometryBiochemical screening or genetic disorders,Drug Monitoring and Toxicology,Vitamins and Related Metabolites,Analysis of Steroid Hormones